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KMID : 0894520110150040301
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Development & Reproduction
2011 Volume.15 No. 4 p.301 ~ p.308
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Effects of Concentration and Immersion Time of Three Cryoprotective Agents on the Embryos Development of Abalone Haliotis discus hannai
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Chung Jong-Kyun
Chang Young-Jin
Lim Han-Kyu
Son Maeng-Hyun
Kim Jong-Hyun
Jeong Min-Hwan
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Abstract
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The tolerance evaluation for abalone Haliotis discus hannai embryos was performed using different concentrations of cryoprotective agents (CPAs): dimethyl sulfoxide, ethylene glycol and propylene glycol added to 0.2 M sucrose, respectively. 4-cell, trochophore and veliger were exposed in each CPA with different concentration for 10, 20 and 30 minutes of immersion time. Developmental rates were increased with decreased concentration of every CPA and decreased immersion time, and differed from types of CPA. Developmental rates of veliger in all the CPAs were higher than those of 4-cell and trochophore. The developmental rates and larval activity indices in ethylene glycol were comparatively higher than those in other CPAs and the effective CPA and its concentration for the cryopreservation of the abalone embryos was suggested as 2.0 M ethylene glycol with equilibration time of 30 minutes.
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KEYWORD
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Abalone, Haliotis discus hannai, Embryo, Development, CPA, Immersion test, Cryopreservation
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